SERVICES

Overview of Services

  Global Profiling Targeted Analysis
  Advanced Scan Basic Scan Dual Scan Mediator Scan C-SCOPE F-SCOPE
Target +Unknown*1 metabolites Primary metabolic pathways +Lipid metabolism Lipid mediators Central energetic metabolism Carbon & Amino acid turn-over
Coverage Central carbon metabolism  
Protein & DNA
turn-over
 
(optional choice)
Other primary metabolism      
Lipid profiling        
“Unknown” biomarkers          
Our Library 1,500 +
unknown peaks*1
900 1,200 400 116
+ 30 parameters*2
54
+ isotopmers*3
Platforms CE-TOFMS CE-TOFMS
LC-TOFMS
LC-MS/MS CE-TOF/QqQ MS CE-TOFMS
Reporting value Relative values
(Quantitation option is available)
Absolute quantitation Relative values
+ Quantitation OP
Data analysis + Marker Screening Statistical analysis, PCA, HCA, Pathway mapping
(*depending on study design)
Isotope distribution
Applications Biomarker Biomarker / Fingerprinting / MOA MOA
  • *1 Unknown peaks mean metabolite without corresponding detection profiles in our compound library.
  • *2 Biochemical parameters are calculated from detection values and represents cellular status.PDF
  • *3 Targets are selected from i) 30 carbon-flux metabolites, and/or ii) 24 amino acid-flux metabolites.PDF

Please contact HMT for more information.

Global Profiling

Metabolomics, a comprehensive analysis of small molecular compounds in biological samples, is a powerful tool because almost all biological phenomena result in changes in an organismal or cellular metabolic state. However, grasping global changes in metabolism is not easy because it requires the precise measurement of these compounds with a variety of physical and chemical properties.

We provide a broad profiling of hydrophilic metabolites with our Capillary Electrophoresis Mass Spectrometric (CE-MS) platforms, adding additional hydrophobic metabolites with our LC-MS platform to capture a wide variety of metabolites and pathways.

Basic Scan / Dual Scan – Multipurpose Profiling for Global Metabolism

Basic Scan / Dual Scan - Multipurpose Profiling for Global Metabolism

Global metabolic profiling targets more than 1,200 metabolites involved in primary and secondary metabolism pathways shared by many organisms.

Our unique CE-MS analysis provides accurate and robust profiling including the metabolism of sugars, amino acids, nucleotides, lipids, and stress response biomarkers. Together with CE-MS, LC-MS based lipidomics provides changes in lipid turnover engaged in cellular energy and signaling pathways.

This package is useful for researchers in a variety of areas and sample species.

  • Global measurement of > 1,200 metabolites involved in primary and secondary metabolism Basic Scan: CE-MS analysis of sugars, amino acids, nucleotides and other ionic metabolites Dual Scan: LC-MS analysis of fatty acids, steroids and other lipids in addition to Basic Scan
  • Report including pathway mapping, PCA, HCA and statistical analysis
  • Suitable for a variety of research areas including medical, agriculture, bioprocessing, etc.

Advanced Scan – Ultimate Metabolomics for Biomarker Discovery

Advanced Scan - Ultimate Metabolomics for Biomarker Discovery

Our Advanced profiling mode covers the complete metabolome including unknown metabolites observed in CE-TOFMS data analysis. In addition to cell derived metabolites, peptides, exogenous compounds, and disease specific novel compounds will be detected and screened as biomarkers for disease or bioprocessing.

All projects are performed under QC conditions based on established SOPs and experience of over 10 years of operation. Together with multivariate and statistical analysis, this package is the ultimate tool for biomarker screening and metabolism based profiling.

  • Profiling of whole compounds measured by CE-MS including unknown metabolites
  • Unknown compounds accompany precise m/z assisting the prediction of chemical structure
  • Report including pathway mapping, PCA, HCA, statistical analysis and follow up discussion
  • Suitable for biomarker screening and metabolome based profiling

Mediator Scan – Targeted for Signaling and the Inflammasome

Mediator Scan - Targeted for Signaling and the Inflammasome

In contrast to the LC-MS method for Dual Scan, M-SCAN represents a novel targeted method for measuring lipid mediators, critical signaling modulators involved in many pathways such as inflammation, allergic response, oxidative stress, and the various metabolic diseases.

The sample protocol for M-SCAN also minimizes large triglycerides in favor of 400 lipid mediators. While the current protocol is designed for relative expression, quantitative profiling for selected lipids can be requested by our clients.

M-Scan Targets Include:

  • Fatty acid-derived – prostaglandins, thromboxanes, leukotrienes, lipoxins etc.
  • Phosphate structures – platelet activating factors, endogenous cannabinoids (anandamide, 2-acylglycerol), lysophosphatidic acids, lysophotidylcholines, lysophosphatidylserines, sphingosine-1-phosphates etc.
  • Sterol structures – glucocorticoids (glucose corticoid), aldosterones (mineral corticoid), sex steroids (estrogen, androgen etc.), bile acids, vitamin D, etc.

Lipid mediators have a wide variety of effects on physiological functions, including immunity, biological defense, blood-pressure regulation, pain or fever, gastrointestinal tract activity and growth, division, and differential regulation of cells. Many diseases are associated with failure in functional balance of such effects. A large portion of lipid mediators act by binding with G-protein coupled receptors found on the cell membrane. Meanwhile, steroid hormones, vitamin A, vitamin D, and others bind with nuclear receptors and induce gene expression changes.

Optional Analysis

  • Absolute quantitation of 110 target metabolites for CE-MS analysisPDF
  • LC-MS analysis for animal lipidomics
  • CE-MS analysis for short peptides
  • Additional data analysis (PCA, HCA, pathway mapping etc.)
  • Feasibility experimental test for sample assessment

Targeted Assays

Central metabolism, which includes bioenergetics and protein / nucleotide turnover is involved in most cellular systems, and therefore is regarded as core to understanding systems biology. Recent research has revealed the importance of primary metabolism in several areas including oncology, stem cell biology, bioprocessing etc.

By using our original CE-MS technology, we provide accurate measurements of central metabolism with absolute quantitation.
The analysis report includes multivariate analysis, metabolic pathway mapping and absolute quantitation based biochemical parameters, which enable the easy monitoring of intracellular metabolic status. Our technology is central to the elucidation of metabolic based drug mechanisms of action. Our technology can promote your investigations about mode of actions based on metabolic pathways.

C-SCOPE / CARCINOSCOPE – Absolute Quantitative Analysis of Energy Metabolism

C-SCOPE / CARCINOSCOPE

Central metabolism, which includes sugars, amino acids, nucleotides and lipids, is involved in the many metabolic systems including bioenergetics, stress response and turnover of cellular components such as protein and nucleic acids.
Our unique CE-MS technology is the only platform to provide accurate measurement for such intracellular intermediates. With absolute quantitation of 116 target metabolites engaged in central metabolism, we will provide basic biochemical parameters which enable researchers to better understand cellular status such as bioenergetics, oxidative stress, mitochondrial function etc.
This package is a high-value tool for the investigation of metabolism of cancer and other metabolic diseases.

List of 116 targeted metabolites

  • 116 target metabolites involved in sugar, protein, DNA/RNA and lipid metabolismPDF
  • 30 biochemical parameters monitoring intracellular statusPDF
  • Absolute quantitation with pathway mapping and multivalent analyses

F-SCOPE– 13C Isotope Labeling Analysis

F-SCOPE

The determination of metabolic flux is a critical component of metabolism research, however, in the past it has been difficult extracting meaningful data due to lags in technology development.
HMT provides quantitative isotopic studies using CE-MS technology for a deeper understanding of intracellular metabolic flux.
By using multiple labeled substrates, such as glucose or glutamine, we can effectively provide a clear picture of central energy metabolism.
Together with our experiences in medical and healthcare research, this package is your first step for the estimation of metabolic flux.

List of F-Scope targeted metabolites

  • 54 target metabolite involved in glycolysis, TCA cycle, PPP and amino acid turnoverPDF
  • Absolute quantitation and distribution of labeled isotopmers
  • Assist of study design and data interpretation

Flux Analysis

Progress to understanding in vivo metabolomics: C13 Isotope studies.

One key to understanding cellular metabolomics lies in the ability to accurately determine the specific metabolic pathways that are employed. Steady state metabolomics is an easy way to initially understand the metabolic needs of specific cell lines and gene mutations.

C13 isotope analyses are a very useful tool for following the fates of nutrients inside the cell. In vitro experiments are easy to perform and can yield valuable insights. The push towards in vivo C13 labeling will allow researchers to confirm the cells preferred metabolic pathways and better understand druggable targets.

HMT is committed to providing our unique C13 analyses for cultured cells, as well as, in the in vivo setting.

C13 Isotope Tracing

  • Use C13 substrates to determine nutrient processing
  • Uncover novel mechanisms for cell survival or apoptosis

More Information

Lipidomics

Divide and Conquer for Optimum Coverage

Although CE-MS has proven to be the best technology for profiling hydrophilic and charged metabolites, CE-MS is not as useful for the metabolic profiling of more hydrophobic and lipid metabolites such as longer chain fatty acids, neutral steroids, and lipids. The profiling of these hydrophobic compounds requires the use of reverse phase HPLC – MS methods and different extraction protocols.
At HMT we provide multiple metabolite extractions to optimize for the detection of a wide range of metabolites in multiple sample types. This allows HMT to produce complex and comprehensive data files for biomarker discovery and patient stratification as part of our DEEP DIVE Platform.

Dual Scan – Untargeted and Unbiased

Our Dual Scan is the combination of choosing one of our CE-MS options (Basic Scan, Advanced Scan, Deep Dive) with an untargeted LC-MS method to capture a wide range of lipids (see Figure). Samples are partitioned into two different extractions. The LC-MS extraction excludes larger triglycerides, in favor of smaller lipids – steroids, long chain fatty acids, bile acids, acyl carnitines, and much more. This untargeted approach is great for novel research, biomarker discovery, clinical analysis and works across multiple sample types.

What is the difference between HMT’s Dual Scan and typical LC-MS based metabolomics discovery?

A majority of LC-MS based platforms focus on a compromised protocol designed to capture a broad range of hydrophilic and hydrophobic metabolites. At HMT, we separate the charged and hydrophilic metabolites from hydrophobic metabolites by using two different extractions and two methods, CE-MS and LC-MS. By using this approach, we optimize the sensitivity, dynamic range and depth of analysis of hydrophilics at the same time as hydrophobics.

M-Scan – Targeted for Signaling and the Inflammasome

In contrast to the LC-MS method for Dual Scan, M-SCAN represents a novel targeted method for measuring lipid mediators, critical signaling modulators involved in many pathways such as inflammation, allergic response, oxidative stress, and the various metabolic diseases.
The sample protocol for M-SCAN also minimizes large triglycerides in favor of 400 lipid mediators. While the current protocol is designed for relative expression, quantitative profiling for selected lipids can be requested by our clients.

M-Scan Targets Include:

  • Fatty acid-derived – prostaglandins, thromboxanes, leukotrienes, lipoxins etc.
  • Phosphate structures – platelet activating factors, endogenous cannabinoids (anandamide, 2-acylglycerol), lysophosphatidic acids, lysophotidylcholines, lysophosphatidylserines, sphingosine-1-phosphates etc.
  • Sterol structures – glucocorticoids (glucose corticoid), aldosterones (mineral corticoid), sex steroids (estrogen, androgen etc.), bile acids, vitamin D, etc.

Lipid mediators have a wide variety of effects on physiological functions, including immunity, biological defense, blood-pressure regulation, pain or fever, gastrointestinal tract activity and growth, division, and differential regulation of cells. Many diseases are associated with failure in functional balance of such effects. A large portion of lipid mediators act by binding with G-protein coupled receptors found on the cell membrane. Meanwhile, steroid hormones, vitamin A, vitamin D, and others bind with nuclear receptors and induce gene expression changes.

Lipidomics