C-SCOPE

Highly sensitive quantitative analysis of central energy metabolism

Highly sensitive analysis of 116 hydrophilic and ionic metabolites involved in central energy metabolism

Absolute quantitative analysis with three-point calibration

Metabolic pathway mapping, PCA, and HCA with heat map are included

C-SCOPE provides quantitative analysis of hydrophilic and ionic metabolites involved in central energy metabolism (glycolysis, TCA cycle, amino acid metabolism, and nucleic acid metabolism) with high sensitivity and accuracy.
It covers 116 metabolites, including sugar phosphates, amino acids, and purine metabolites. In addition, all detected metabolites are subject to absolute quantification with three-point calibration.
C-SCOPE is ideal for those who wish to compare pool sizes of metabolites and calculate metabolic parameters, such as the energy charge of nucleic acids, as it can quantitatively determine the metabolic states of living organisms and cells.

Results will be summarized in a study report, with an Excel file containing numerical data.

The report will include the following:

  • Concentrations of 116 target metabolites in the samples
  • Principal component analysis (PCA) and heat map of hierarchical clustering analysis (HCA) results
  • Pathway maps drawn based on the measurement results, starting from the central energy metabolism
  • 30 metabolic parameters derived from the concentration of each metabolite

Analysis Details

Plan Overview HMT’s CE-MS platform, which combines capillary electrophoresis (CE) and mass spectrometry (MS), provides comprehensive analysis of metabolites i.e., metabolomics analysis. CE’s high resolution is ideal for the profiling of hydrophilic and ionic metabolites that are abundant in living organisms. Moreover, it enables the detection of metabolites that are difficult to separate with other platforms.
Main Applications Mechanistic analysis that focuses on central energy metabolism.
Research that requires stable analysis over a long period of time.
Target Metabolites
  • 116 metabolites engaged in central energy metabolism
  • Glycolysis/gluconeogenesis, TCA cycle, pentose phosphate pathway, lipid metabolism, amino acid metabolism, metabolites related to nucleic acid metabolism, etc.
Sample Type Animal tissues (liver, muscle, brain), cultured cells, animal blood, culture medium, cultured microbial cells (yeast, E. coli)
Platform CE-QqQMS
CE-TOFMS
Standard Turnaround Time Within 60 days

Frequently Asked Questions

What are “metabolic parameters”?

Metabolic parameters enable researchers to better understand cellular status such as bioenergetics, oxidative stress, mitochondrial function, etc. The metabolic parameters that are featured in the report include adenylate energy charge, an indicator of intracellular energy status; Fischer’s Ratio, an indicator of liver function; LP Ratio, an indicator of mitochondrial function; and total amount of various amino acids. These parameters can only be calculated when the absolute concentrations of metabolites are available.

 

What sample types can be analyzed?

Mainly cultured cells, animal tissues and blood.

 

Is there a minimum order requirement?

We accept a minimum of 6 samples for C-SCOPE. Bulk discount is available depending on the number of samples. Please contact us for details.

 

Can you quantify the concentration of metabolites?

C-SCOPE reports quantitative concentration values for all target metabolites.

 

What are the benefits of knowing the concentration?

By knowing the concentration, the following can be performed:

  • Comparison between different metabolites
  • Addition or subtraction of certain metabolites (e.g., for calculation of total amino acids or branched chain amino acids, etc.)
  • Comparison of data from different batches

Thus, making it possible to calculate metabolic parameters and integrate the results with past data.

 

What are the metabolites covered by C-SCOPE?

Please refer to our Annotation List for the lineup of measurable metabolites.

 

Is fatty acid metabolism included in the analysis?

Fatty acids are not included in the C-SCOPE analysis as they are lipophilic metabolites. However, combining it with LC-TOFMS Analysis allows fatty acids and acylcarnitines to be added to the measurement targets.

 

How is C-SCOPE different from Basic Scan?

C-SCOPE provides highly sensitive analysis of 116 metabolites involved in energy metabolism and has the ability to quantify the concentration of these target metabolites.

 

How much difference in sensitivity does CE-QqQMS have compared to CE-TOFMS?

The sensitivity is increased by a factor of 10 to 1,000, depending on the metabolite.

 

How much does the analysis cost?

The price will vary depending on the test scale, number of samples and inclusion of any optional analyses. Please contact your local distributor. If your local distributor is not listed here, please contact us.

 

How do I request for analysis?

Please refer to our Service Flow.

 

Please check here for information on the required sample volume, study design and report.

Service Leaflet : C-SCOPE

For more detailed information, download our Service Leaflet from the following page:

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