Highly sensitive analysis of lipid mediators

Comprehensive analysis of 400 lipophilic metabolites (lipid mediators)

Report includes metabolic pathway mapping based on the measurement results

PCA and HCA with heat map are also included

Mediator Scan provides a comprehensive analysis of 400 lipid mediators, including oxylipins, lysophospholipids, and steroids.
Lipid mediators are involved in a wide range of physiological functions, making them ideal for clarifying the pathogenesis, as well as searching for biomarkers in various diseases such as immune- and inflammation-related diseases (asthma, hives, rheumatism, multiple sclerosis, pulmonary fibrosis, inflammatory bowel disease, atopy, food allergy, etc.) and lifestyle-related diseases (diabetes and atherosclerosis, cancer, dementia).
In addition, Mediator Scan can be used not only as a stand-alone analysis tool, but also in combination with analysis plans for hydrophilic and ionic metabolites, such as Advanced Scan and Basic Scan, for a highly sensitive and comprehensive analysis.

Results will be summarized in a study report, with an Excel file containing numerical data.

The report will include the following:

  • Detected peak values (relative area values)
  • Principal component analysis (PCA) and heat map of hierarchical clustering analysis (HCA) results
  • Pathway maps drawn based on measurement results that include oxylipins, steroids, etc.

Analysis Details

Plan Overview HMT’s LC-MS/MS platform, which combines liquid chromatography (LC) and MS/MS, provides comprehensive analysis of 400 lipid mediators. It is also possible to increase the analysis coverage by combining it with other analysis plans such as Advanced Scan and Basic Scan.
Target Metabolites
  • 1st generation lipid mediators : Eicosanoids [ω6 fatty acid metabolites (prostaglandin, thromboxane, leukotriene)]
  • 2nd generation lipid mediators : Lysophospholipids (LPA, LPC, LPE, LPG, LPI, LPS)
  • 3rd generation lipid mediators : EPA/DHA metabolites [ω3 fatty acid metabolites (lipoxin, resolvin, protectin)]
  • Other lipid mediators : Platelet-activating factor (PAF), endogenous cannabinoids, sphingosine-1 phosphate, ganglioside, ceramide
Sample Type Blood (plasma*, serum), culture medium/supernatant
* Anticoagulants that are compatible with the analysis are EDTA 2Na, EDTA 2K and sodium citrate.
Platform LC-MS/MS
Standard Turnaround Time Within 90 days

Frequently Asked Questions

What sample types can be analyzed?

Blood (plasma, serum), culture medium or supernatant.


Is there a minimum order requirement?

We accept a minimum of 10 samples for Mediator Scan. Bulk discount is available depending on the number of samples. Please contact us for details.


Can you quantify the concentration of metabolites?

Absolute concentration of metabolites is not available for Mediator Scan. All data are reported in relative area values.


What are the metabolites covered by Mediator Scan?

Please refer to our Annotation List for the lineup of measurable metabolites.


How is Mediator Scan different from LC-TOFMS analysis?

In Mediator Scan, lipophilic metabolites are classified into the different molecular species or classes. It is designed specifically for lipid mediators, covering 400 metabolites including eicosanoids/docosanoids, lysophospholipids, sphingolipids, and platelet-activating factor.

On the other hand, LC-TOFMS analysis covers a wide but shallow range of around 300 lipophilic metabolites, including simple/complex lipids, fatty acids, bile acids, steroids, and polyphenols.


How much does the analysis cost?

The price will vary depending on the test scale, number of samples and inclusion of any optional analyses. Please contact your local distributor. If your local distributor is not listed here, please contact us.


How do I request for analysis?

Please refer to our Service Flow.


Please check here for information on the required sample volume, study design and report.

Service Leaflet : Mediator Scan

For more detailed information, download our Service Leaflet at the following page: