What is stable isotope-labeling analysis?
Isotopes are variants of elements commonly found in nature that have different atomic masses. When a substrate molecule containing an isotope is metabolized and converted into a different metabolite, the isotope atom remains intact, allowing it to be used as a “label”. Molecules containing stable isotopes have different mass-to-charge ratio (m/z) than normal molecules and can be distinguished by mass spectrometry. Therefore, it is possible to use isotope labels to analyze which pathways are used to metabolize the substrates and what metabolites are formed from these substrates.
What kind of label can be used?
F-SCOPE typically analyzes 13C, however, it may be possible to analyze other labels. Please contact us for details.
What sample types can be analyzed?
Isotope-labeling analysis requires efficient incorporation of the labeled substrate into the samples, therefore, cells from animals, plants, and microorganisms, are typically used.
Is there a minimum order requirement?
We accept a minimum of 12 samples for F-SCOPE. Bulk discount is available depending on the number of samples. Please contact us for details.
Can you quantify the concentration of metabolites?
F-SCOPE reports quantitative concentration values for all target metabolites.
What are the metabolites covered by F-SCOPE?
Please refer to our Annotation List for the lineup of measurable metabolites.
How much does the analysis cost?
The price will vary depending on the test scale, number of samples and inclusion of any optional analyses. Please contact your local distributor. If your local distributor is not listed here, please contact us.
How do I request for analysis?
Please refer to our Service Flow.
Please check here for information on the required sample volume, study design and report.