HMTは、第72回日本癌学会学術総会（2013年 10月 3日 – 10月 5日）に出展いたします。
- 日時
- 2013年 10月 3日（木）～ 10月 5日（土）
- 会場
- パシフィコ横浜 (神奈川県横浜市)
- 参加費
- 12,000 円 (会員、事前登録)
HMTはブース#25で展示いたします。HMTのブースにお立ち寄りいただいた方には来場特典がございます。ぜひお立ち寄りください。
研究員の紙が口頭発表を行います
（E-2070）
CE-QqQMSによる高感度メタボローム解析手法の開発および分化型・未分化型胃がんプロファイリングへの応用
CE-QqQMS-based Metabolomics Discriminated Undifferentiated and Differentiated Gastric Cancer
- セッション
- E11-5 メタボローム (1)／Metabolome (1)
- 日時
- 2013/10/4 13:00～14:00
- 会場
- Room 8 411+412
- 演者
- 紙 健次郎、佐々木 一謹、藤森 玉輝、寺島 雅典、楠原 正俊、徳永 正則、谷澤 豊、坂東 悦郎、川村 泰一、山本 博之、大橋 由明
- 要旨
- Most metabolites involved in the primary energy metabolism are anions; however, their concentrations in cells and tissues are often too low to be measured accurately by conventional metabolomics platforms. Here, we developed a capillary electrophoresis-triple quadrupole mass spectrometry (CE-QqQMS)-based metabolomics technique, which dramatically improved detection sensitivity of 63 anions up to 291-folds compared to a conventional analysis. The method was then applied to characterize metabolomic profiles of paired non-tumor and tumor tissues of 27 gastric cancer patients. The results not only distinguished their profiles but also delineated differentiation-status-dependent tumor profiles: Differentiated tumor (DT) tissues showed considerably high glycolytic intermediate, nucleotide, and glutathione levels, whereas undifferentiated tumor (UT) tissues rather exhibited relatively low energy and redox statuses, which were also observed in DT and UT cell lines. Taken together, DT exhibited more typical cancer metabolic profiles than UT represented by active Warburg- and amino acid-dependent metabolism, whereas low glutathione ratios in UT imply their vulnerability to oxidative stress.
- キーワード
- Gastric cancer, metabolomics
共同研究している静岡がんセンター胃外科 徳永 正則先生が口頭発表を行われます
（E-2072）
食道癌組織におけるメタボローム解析
Metabolome analysis of esophageal cancer tissues using capillary electrophoresis time-of-flight mass spectrometry
- セッション
- E11-6 がん細胞の特性／Characteristics of cancer cells
- 日時
- 2013/10/4 15:00～16:00
- 会場
- Room 8 411+412
- 演者
- 徳永 正則、小澤 壯治、紙 健次郎、宮地 勇人、林 勉、安田 聖栄、大橋 由明、藤森 玉輝、星 綾、楠原 正俊、寺島 雅典
- 要旨
- Backgrounds: Metabolomic characteristics of esophageal cancer have been largely unknown. We thus conducted metabolome analysis of paired non-tumor (NTs) and tumor (Ts) esophageal tissues by using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS).Methods: Tumor and surrounding non-tumor tissues were surgically excised pair-wise from 10 esophageal cancer patients. Following tissue homogenization and metabolite extraction, we measured 235 metabolites by CE-TOFMS.Results: Principal component analysis showed clear metabolomic differences between NTs and Ts. Lactate and citrate levels in Ts were significantly higher and lower, respectively, than those in NTs, which reflects hypoxic microenvironment and corroborates the Warburg effect in Ts. Concentrations of most amino acids except Gln were higher in Ts than in NTs presumably due to accelerated glutaminolysis and autophagic protein degradation in Ts.Conclusion: The hallmarks of cancer metabolism were reassured by comparing metabolomic profiles of esophageal NTs and Ts. Several metabolites were detected only in tumor tissues, which may lead to elucidate tumor-specific metabolic pathways.
- キーワード
- Metabolome
大会の詳細は第72回日本癌学会学術総会ウェブサイトをご覧ください。