2nd Symposium on Complex Biodynamics & Networks

HMTは山形県鶴岡市で開催される2nd Symposium on Complex Biodynamics & Networksにて出展およびランチョンセミナーを行います。

概要

ランチョンセミナー

Development and Applications of Quantitative and Isotope-based Metabolomics for Life Sciences and Systems Biology

演者

紙 健次郎
ヒューマン・メタボローム・テクノロジーズ株式会社

日時

2015年5月12日（火）12時45分～13時15分

参加費

無料（お弁当付き）

要旨

Metabolomics technology has been highly sophisticated in recent years for its applications in metabolic profiling, biomarker discovery, drug development, and systems biology. However, most omics technologies are limited to providing relative quantification, the data of which by default cannot be accumulated and collectively evaluated. In addition, concentrations of most metabolites involved in primary energy metabolism such as glycolysis, pentose phosphate pathway, and the TCA cycle, are often too low to be detected in cells and tissues by using conventional metabolomics platforms. Here, we developed a capillary electrophoresis-triple quadrupole mass spectrometry (CE-QqQMS)-based metabolome analysis package, or C-SCOPE, in which 116 metabolites in the primary energy metabolism are absolutely quantified with up to 291-fold higher detection sensitivities than those of conventional time-of-flight mass spectrometry-based approach. Recent applications of C-SCOPE will be introduced, including metabolic profiling of yeast harboring a mutant glycolytic gene, cultured cells treated by molecular-targeted anticancer drugs, and liver tissues of food-deprived mice. In addition, absolute quantification of isotopomers is also crucial for clarifying the direction of metabolic flow from an isotopically-labeled compound and conducting stoichiometric flux analysis. We released a quantitative isotopomer analysis package, or F-SCOPE, providing absolute concentrations for a series of isotopomers of 54 metabolites comprising intermediates in the central carbon metabolism, amino acids, glutathiones, and purine nucleotides, which is thus suited, for example, to trace 13C-labeled glucose for optimizing fermentation conditions or 13C-Gln for disturbing glutaminolysis in cancer cells. Taken together, our quantitative metabolome analysis packages, C-SCOPE and F-SCOPE, enable researchers to develop big data, which can be analyzed comprehensively and computationally for elucidating the energy metabolism in complex biological systems.

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